Abn2

From SubtiWiki
Revision as of 13:40, 20 February 2014 by Raphael2215 (talk | contribs)
Jump to: navigation, search
  • Description: endo-1,5-alpha-L-arabinosidase

Gene name abn2
Synonyms yxiA
Essential no
Product endo-1,5-alpha-L-arabinosidase
Function arabinan degradation
Gene expression levels in SubtiExpress: abn2
MW, pI 52 kDa, 7.371
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours yxiB, yxzL
Sequences Protein DNA DNA_with_flanks
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
YxiA context.gif
Expression at a glance   PubMed
Abn2 expression.png



Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. PubMed
  • Domains:
    • N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown PubMed
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number: 3.2.1.99

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (4.3-fold) (CcpA) PubMed
    • expression is stimulated by arabinose and pectin and repressed by glucose PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabel de Sa-Nogueira, Lisboa, Portugal homepage

Your additional remarks

References