MalA

• Description: 6-phospho-alpha-glucosidase
  
  

Gene name	malA
Synonyms	glvG, glvA
Essential	no
Product	6-phospho-alpha-glucosidase
Function	maltose utilization
Gene expression levels in SubtiExpress: malA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	50 kDa, 4.74
Gene length, protein length	1347 bp, 449 aa
Immediate neighbours	yfjA, glvR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, GlvR regulon

The gene

Basic information

• Locus tag: BSU08180

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Maltose 6'-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 4 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane associated PubMed

Database entries

• Structure: 1U8X

• UniProt: P54716

• KEGG entry: [3]

• E.C. number: 3.2.1.122

Expression and regulation

• Operon: malA-glvR-malP PubMed

• Expression browser: malA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induction by maltose (GlvR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GlvR: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Yang M Ming, Zhang W Wei, Chen Y Lin, Gong Y Sheng
Development of a Bacillus subtilis expression system using the improved Pglv promoter.
Microb Cell Fact: 2010, 9;55
[PubMed:20618987] [WorldCat.org] [DOI] (I e)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Vivian L Y Yip, John Thompson, Stephen G Withers
Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4.
Biochemistry: 2007, 46(34);9840-52
[PubMed:17676871] [WorldCat.org] [DOI] (P p)

Stefan Schönert, Sabine Seitz, Holger Krafft, Eva-Anne Feuerbaum, Iris Andernach, Gabriele Witz, Michael K Dahl
Maltose and maltodextrin utilization by Bacillus subtilis.
J Bacteriol: 2006, 188(11);3911-22
[PubMed:16707683] [WorldCat.org] [DOI] (P p)

Shyamala S Rajan, Xiaojing Yang, Frank Collart, Vivian L Y Yip, Stephen G Withers, Annabelle Varrot, John Thompson, Gideon J Davies, Wayne F Anderson
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+ -dependent phospho-alpha-glucosidase from Bacillus subtilis.
Structure: 2004, 12(9);1619-29
[PubMed:15341727] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Serizawa, J Thompson, J Sekiguchi
Regulation of the glv operon in Bacillus subtilis: YfiA (GlvR) is a positive regulator of the operon that is repressed through CcpA and cre.
J Bacteriol: 2001, 183(17);5110-21
[PubMed:11489864] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

J Thompson, A Pikis, S B Ruvinov, B Henrissat, H Yamamoto, J Sekiguchi
The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily.
J Biol Chem: 1998, 273(42);27347-56
[PubMed:9765262] [WorldCat.org] [DOI] (P p)