FolC

• Description: folyl-polyglutamate synthetase
  
  

Gene name	folC
Synonyms
Essential	no
Product	folyl-polyglutamate synthetase
Function	biosynthesis of folate
Gene expression levels in SubtiExpress: folC
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Folate
MW, pI	48 kDa, 5.806
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	comC, valS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, membrane proteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU28080

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + tetrahydropteroyl-(gamma-Glu)(n) + L-glutamate = ADP + phosphate + tetrahydropteroyl-(gamma-Glu)(n+1) (according to Swiss-Prot)

• Protein family: folylpolyglutamate synthase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1FGS (from Lactobacillus casei, 32% identity, 51% similarity) PubMed

• UniProt: Q05865

• KEGG entry: [3]

• E.C. number: 6.3.2.17

Expression and regulation

• Operon: valS-folC PubMed

• Expression browser: folC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by valine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: riboswitch, transcription termination/antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

D Luo, J Leautey, M Grunberg-Manago, H Putzer
Structure and regulation of expression of the Bacillus subtilis valyl-tRNA synthetase gene.
J Bacteriol: 1997, 179(8);2472-8
[PubMed:9098041] [WorldCat.org] [DOI] (P p)