SacX

• Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component of the PTS (low affinity) and control of SacY activity
  
  

Gene name	sacX
Synonyms	ipa-14r, sacS
Essential	no
Product	trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
Function	sucrose uptake and phosphorylation, control of SacY activity
Gene expression levels in SubtiExpress: sacX
Interactions involving this protein in SubtInteract: SacX
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	48 kDa, 9.346
Gene length, protein length	1377 bp, 459 aa
Immediate neighbours	epr, sacY
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, transcription factors and their control, trigger enzyme, membrane proteins, phosphoproteins

This gene is a member of the following regulons

SacY regulon

The gene

Basic information

• Locus tag: BSU38410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)

• Protein family: PTS permease, sucrose permease (Scr) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P15400

• KEGG entry: [3]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: sacX-sacY PubMed

• Expression browser: sacX PubMed

• Sigma factor: SigA PubMed

• Regulation: induction by sucrose (at high concentration) PubMed

• Regulatory mechanism:
  • induction by SacY-dependent RNA switch (transcriptional antitermination) PubMed
  • DegU-P: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: