ThyB

• Description: thymidylate synthase B
  
  

Gene name	thyB
Synonyms
Essential	no
Product	thymidylate synthase B
Function	biosynthesis of thymidine nucleotides
Gene expression levels in SubtiExpress: thyB
Interactions involving this protein in SubtInteract: ThyB
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	30 kDa, 6.272
Gene length, protein length	792 bp, 264 aa
Immediate neighbours	dfrA, ypjQ
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins

The gene

Basic information

• Locus tag: BSU21820

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-102 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DfrA-ThyB

• Localization:

Database entries

• Structure:

• UniProt: P11044

• KEGG entry: [2]

• E.C. number: 2.1.1.45

Expression and regulation

• Operon: thyB-dfrA PubMed

• Expression browser: thyB PubMed

• Sigma factor:

• Regulation:
  • weak induction in the presence of glucose (CcpN) PubMed

• Regulatory mechanism:
  • CcpN: weak transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: