DnaC

• Description: replicative DNA helicase, part of the replisome
  
  

Gene name	dnaC
Synonyms
Essential	yes PubMed
Product	replicative DNA helicase
Function	DNA replication
Gene expression levels in SubtiExpress: dnaC
Interactions involving this protein in SubtInteract: DnaC
MW, pI	50 kDa, 4.548
Gene length, protein length	1362 bp, 454 aa
Immediate neighbours	yycE, yycD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA replication, essential genes

The gene

Basic information

• Locus tag: BSU40440

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • stimulates DnaG primase activity PubMed

• Protein family: DnaB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DnaB-DnaC PubMed
  • DnaI-DnaC PubMed
  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • YqaM-DnaC PubMed
  • part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed
  • DnaX-DnaC PubMed
  • DnaE-DnaC PubMed
  • DnaG-DnaC PubMed

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure: 2R6A (Geobacillus stearothermophilus), 2R6C, 2R6D, 2R6E

• UniProt: P37469

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dnaC (according to DBTBS)

• Expression browser: dnaC PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Geoffrey S Briggs, Wiep Klaas Smits, Panos Soultanas
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.
J Bacteriol: 2012, 194(19);5162-70
[PubMed:22797751] [WorldCat.org] [DOI] (I p)