GalE

• Description: UDP glucose 4-epimerase
  
  

Gene name	galE
Synonyms
Essential	no
Product	UDP glucose 4-epimerase
Function	galactose utilization
Gene expression levels in SubtiExpress: galE
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	36 kDa, 4.776
Gene length, protein length	1017 bp, 339 aa
Immediate neighbours	yxkC, yxkA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, biofilm formation

The gene

Basic information

• Locus tag: BSU38860

Phenotypes of a mutant

• • smooth colonies on MsGG medium, no biofilm formation in the absence of externally provided galactose PubMed
  • the mutant is sensitive to exogenous galactose due to the accumulation of toxic UDP-galactose, this sensitivity can be suppressed by the inactivation of galK (no formation of the toxic UDP-galactose) or of sinR (constitutive expression of the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO results in detoxification of UDP-galactose) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-glucose = UDP-galactose (according to Swiss-Prot)

• Protein family: sugar epimerase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1LRL (from Escherichia coli Y299C mutant, 57% identity, 71% similarity) PubMed

• UniProt: P55180

• KEGG entry: [3]

• E.C. number: 5.1.3.2

Expression and regulation

• Operon: galE PubMed

• Expression browser: galE PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed