MaeA

• Description: malic enzyme
  
  

Gene name	maeA
Synonyms	ywkA
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	63 kDa, 4.883
Gene length, protein length	1746 bp, 582 aa
Immediate neighbours	ywkB, tdk
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU37050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate --> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MalS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45868

• KEGG entry: [3]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon: maeA-ywkB PubMed

• Expression browser: maeA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of malate (MalR) PubMed

• Regulatory mechanism:
  • MalR: transcription activation PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant: GP1141 (aphA3) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J Bacteriol: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160] [WorldCat.org] [DOI] (P p)