HutH

• Description: histidase
  
  

Gene name	hutH
Synonyms
Essential	no
Product	histidase
Function	histidine utilization
MW, pI	55 kDa, 5.221
Gene length, protein length	1524 bp, 508 aa
Immediate neighbours	hutP, hutU
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-histidine = urocanate + NH₃ (according to Swiss-Prot)

• Protein family: PAL/histidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P10944

• KEGG entry: BSU39350

• E.C. number: 4.3.1.3

Expression and regulation

• Operon: hutP-hutH-hutU-hutI-hutG-hutM PubMed

• Sigma factor: SigA

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1114 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Fisher SH, Magasanik B (1984) 2-Ketoglutarate and the regulation of aconitase and histidase formation Bacillus subtilis. J Bacteriol 158:379-382. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed