MutY

• Description: A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches
  
  

Gene name	mutY
Synonyms	yfhQ
Essential	no
Product	A/G-specific adenine glycosylase
Function	DNA repair
Gene expression levels in SubtiExpress: mutY
Metabolic function and regulation of this protein in SubtiPathways: mutY
MW, pI	41 kDa, 6.044
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	yfhP, yfhS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

YfhP regulon

The gene

Basic information

• Locus tag: BSU08630

Phenotypes of a mutant

• increased susceptibility to Cr(VI) due to the accumulation of oxidative DNA damage PubMed

Database entries

• BsubCyc: BSU08630

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: releases adenines from 8-oxo-G:A mismatches

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08630

• Structure: 1RRS (complex with DNA, Geobacillus stearothermophilus)

• UniProt: O31584

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutY-fabL-sspE PubMed

• Expression browser: mutY PubMed

• Sigma factor:
  • SigA PubMed
  • SigG PubMed

• Regulation:
  • expression is constitutive throughout growth PubMed
  • expression is fivefold increased in a mutS-mutL mutant PubMed
  • YfhP may act as a negative transcription regulator of the mutY-fabL-sspE operon during exponential growth PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 52 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 168 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 201 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 215 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)