IlvA

• Description: threonine dehydratase
  
  

Gene name	ilvA
Synonyms
Essential	no
Product	threonine dehydratase
Function	biosynthesis of branched-chain amino acids
Gene expression levels in SubtiExpress: ilvA
Metabolic function and regulation of this protein in SubtiPathways: ilvA
MW, pI	46 kDa, 5.538
Gene length, protein length	1266 bp, 422 aa
Immediate neighbours	ypmP, yplP
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

CodY regulon

The gene

Basic information

• Locus tag: BSU21770

Database entries

• BsubCyc: BSU21770

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-threonine = 2-oxobutanoate + NH₃ (according to Swiss-Prot)

• Protein family: ccmF/cycK/ccl1/nrfE/ccsA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU21770

• Structure:

• UniProt: P37946

• KEGG entry: [3]

• E.C. number: 4.3.1.19

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: ilvA-ypmP PubMed

• Expression browser: ilvA PubMed

• Sigma factor:

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 969 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1777 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion: pGP519 (in pAC5), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: