ProH

• Description: pyrroline-5-carboxylate reductase
  
  

Gene name	proH
Synonyms	yoxE
Essential	no
Product	pyrroline-5-carboxylate reductase
Function	osmoadaptive de novo production of proline
Gene expression levels in SubtiExpress: proH
Metabolic function and regulation of this protein in SubtiPathways: proH
MW, pI	31 kDa, 7.754
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	proJ, rtp
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

The gene

Basic information

• Locus tag: BSU18480

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-proline + NAD(P)⁺ = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)

• Protein family: glutamate 5-kinase family (according to Swiss-Prot)

• Paralogous protein(s): ProI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P14383

• KEGG entry: [2]

• E.C. number: 1.5.1.2

Expression and regulation

• Operon: proH-proJ PubMed

• Expression browser: proH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under conditions of osmotic stress PubMed
  • induced by cold stress PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Tamara Hoffmann, Erhard Bremer
Protection of Bacillus subtilis against cold stress via compatible-solute acquisition.
J Bacteriol: 2011, 193(7);1552-62
[PubMed:21296969] [WorldCat.org] [DOI] (I p)

B R Belitsky, J Brill, E Bremer, A L Sonenshein
Multiple genes for the last step of proline biosynthesis in Bacillus subtilis.
J Bacteriol: 2001, 183(14);4389-92
[PubMed:11418582] [WorldCat.org] [DOI] (P p)