HlpB

• Description: HNH nuclease-like protein, rescues AddA-AddB-mediated recombination intermediates
  
  

Gene name	hlpB
Synonyms	yisB
Essential	yes PubMed
Product	HNH nuclease-like protein
Function	rescuing of AddA-AddB-mediated recombination intermediates
Gene expression levels in SubtiExpress: yisB
MW, pI	11 kDa, 9.941
Gene length, protein length	300 bp, 100 aa
Immediate neighbours	sbcC, gerPF
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, essential genes

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU10660

Phenotypes of a mutant

• essential, the mutation can be suppressed by second-site mutations in addA or addB PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • evenly distributed throughout the cytosol in growing cells PubMed
  • forms a single focus on the nucleoid upon induction of DNA damage PubMed

Database entries

• Structure:

• UniProt: O06715

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: addB-addA-sbcD-sbcC-hlpB PubMed

• Expression browser: yisB PubMed

• Sigma factor:

• Regulation:
  • positive regulation by ComK PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: