LcfA

• Description: long chain acyl-CoA synthetase, involved in surfactin production
  
  

Gene name	lcfA
Synonyms
Essential	no
Product	long chain acyl-CoA synthetase
Function	fatty acid degradation
Gene expression levels in SubtiExpress: lcfA
Metabolic function and regulation of this protein in SubtiPathways: Fatty acid degradation
MW, pI	62 kDa, 6.119
Gene length, protein length	1680 bp, 560 aa
Immediate neighbours	fadR, yshE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of lipids

This gene is a member of the following regulons

CcpA regulon, FadR regulon

The gene

Basic information

• Locus tag: BSU28560

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis PubMed

• Protein family: ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

• Paralogous protein(s): LcfB, YhfT, YngI

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P94547

• KEGG entry: [3]

• E.C. number: 6.2.1.3

Expression and regulation

• Operon: lcfA-fadR-fadB-etfB-etfA PubMed

• Expression browser: lcfA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • subject to carbon catabolite repression (CcpA-HPr(Ser-P) PubMed
  • induced by long chain acyl-CoA (C14 ... C20) (FadR) PubMed

• Regulatory mechanism:
  • CcpA-HPr(Ser-P): transcription repression PubMed
  • FadR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: available in Mohamed Marahiel's lab PubMed
  • 1A852 ( lcfA::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed