McsB

• Description: protein arginine kinase, adaptor protein, modulator of CtsR-dependent repression
  
  

Gene name	mcsB
Synonyms	yacI
Essential	no
Product	protein arginine kinase
Function	control of CtsR activity
Gene expression levels in SubtiExpress: mcsB
Interactions involving this protein in SubtInteract: McsB
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	40 kDa, 5.068
Gene length, protein length	1089 bp, 363 aa
Immediate neighbours	mcsA, clpC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

• Locus tag: BSU00850

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: targets non-functional CtsR for degradation by ClpP/ClpC PubMed

• Protein family: ATP:guanido phosphotransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
  • autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • McsA-McsB-CtsR, McsB-CtsR PubMed, McsA-McsB
  • McsB-YwlE PubMed
  • McsB-ClpP/ClpC (rapid degradation of phosphorylated McsB) PubMed

• Localization:

Database entries

• Structure:

• UniProt: P37570

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Expression browser: mcsB PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2, SigF PubMed

• Regulation:
  • expressed early during sporulation in the forespore (SigF) PubMed
  • induced by stress (SigB) PubMed
  • induced by heat (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • mcsB::aphA3 availbale from the Gerth lab
  • mcsBC167S::spec available from the Gerth lab
  • GP1457 (mcsB::aphA3), available in Stülke lab
  • BP69 (spc), available in Fabian Commichau's lab
• Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: available in Gerth lab

Labs working on this gene/protein

• Ulf Gerth, Greifswald, Germany

• Fabian Commichau Göttingen, Germany

References

Reviews

Additional reviews: PubMed

Aurelia Battesti, Susan Gottesman
Roles of adaptor proteins in regulation of bacterial proteolysis.
Curr Opin Microbiol: 2013, 16(2);140-7
[PubMed:23375660] [WorldCat.org] [DOI] (I p)

Original Publications

Additional publications: PubMed