YkuI

• Description: potential c-di-GMP degrading phosphodiesterase
  
  

Gene name	ykuI
Synonyms
Essential	no
Product	potential c-di-GMP degrading phosphodiesterase
Function	unknown
Gene expression levels in SubtiExpress: ykuI
MW, pI	47 kDa, 4.462
Gene length, protein length	1221 bp, 407 aa
Immediate neighbours	ykuH, fsrA
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides

The gene

Basic information

• Locus tag: BSU14090

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: degradation of cyclic di-GMP PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: EAL domain (aa 1 ... 245, for c-di-GMP hydrolysis), PAS-like domain (aa 300 ... 400) PubMed

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2BAS, in complex with c-di-GMP 2W27 PubMed

• UniProt: O35014

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ykuI PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP1324 (tet), available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251] [WorldCat.org] [DOI] (P p)