ClpE

• Description: ATP-dependent Clp protease-like (class III stress gene)
  
  

Gene name	clpE
Synonyms	ykvH
Essential	no
Product	ATP-dependent Clp protease-like
Function	protein degradation
Interactions involving this protein in SubtInteract: ClpE
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	77 kDa, 5.135
Gene length, protein length	2097 bp, 699 aa
Immediate neighbours	motA, ykvI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

proteolysis, heat shock proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

• Locus tag: BSU13700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Additional information

• A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATPase/chaperone

• Protein family: AAA+ -type ATPase (IPR013093) InterPro (PF07724) PFAM

• Paralogous protein(s): ClpC, ClpX

Extended information on the protein

• Kinetic information:

• Domains: AAA-ATPase PFAM

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ClpE-ClpP

• Localization:
  • localization in cytoplasmic polar clusters, excluded from the nucleoid, colocalization with ClpP Pubmed
  • forms foci coincident with nucleoid edges, usually near cell poles PubMed

Database entries

• Structure:

• UniProt: O31673

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: clpE PubMed

• Expression browser: clpE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by heat (CtsR) PubMed

• Regulatory mechanism:
  • CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: clpE::spec available from the Hamoen] Lab

• Expression vector:

• lacZ fusion:

• GFP fusion: C-terminal YFP and CFP fusions (single copy) available from the Hamoen] Lab

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Leendert Hamoen, Newcastle University, UK homepage

References

Reviews

Dorte Frees, Kirsi Savijoki, Pekka Varmanen, Hanne Ingmer
Clp ATPases and ClpP proteolytic complexes regulate vital biological processes in low GC, Gram-positive bacteria.
Mol Microbiol: 2007, 63(5);1285-95
[PubMed:17302811] [WorldCat.org] [DOI] (P p)

Original Publications

Additional publications: PubMed