DisA

• Description: DNA integrity scanning protein, delays sporulation in the case of chromosome damage, has diadenylate cyclase activity
  
  

Gene name	disA
Synonyms	yacK
Essential	no
Product	DNA integrity scanning protein, has diadenylate cyclase activity
Function	control of sporulation initiation
Interactions involving this protein in SubtInteract: DisA
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	40 kDa, 5.569
Gene length, protein length	1080 bp, 360 aa
Immediate neighbours	radA, yacL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell division, metabolism of signalling nucleotides, sporulation/ other, general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigM regulon

The gene

Basic information

• Locus tag: BSU00880

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: synthesizes the small molecule cyclic diadenosine monophosphate (c-di-AMP) PubMed

• Protein family: disA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • RacA-DisA

• Localization:
  • see a video showing the movement of DisA in the cell (in real time) PubMed

Database entries

• Structure: 3C23

• UniProt: P37573

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:
  • ctsR-mcsA-mcsB-clpC-radA-disA PubMed
  • disA PubMed

• Sigma factor:
  • ctsR: SigA PubMed, SigB PubMed1 PubMed2
  • disA: SigM PubMed

• Regulation: induced by stress (SigB) PubMed, induced by heat (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: YA5 disA::tet, available in Stülke lab
  • GP987 (disA::tet), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: