LicA

• Description: lichenan-specific phosphotransferase system, EIIA component of the PTS
  
  

Gene name	licA
Synonyms	celC
Essential	no
Product	lichenan-specific phosphotransferase system, EIIA component
Function	lichenan uptake and phosphorylation
Interactions involving this protein in SubtInteract: LicA
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	12 kDa, 4.641
Gene length, protein length	330 bp, 110 aa
Immediate neighbours	licH, licC
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

CcpA regulon, LicR regulon

The gene

Basic information

• Locus tag: BSU38570

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)

• Protein family: PTS permease, lactose permease (Lac) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • LicA-HPr, LicA-LicB

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3K1S (IIA(Cel) from B. anthracis, 42% identity)

• UniProt: P46319

• KEGG entry: [2]

• E.C. number: 2.7.1.69

Expression and regulation

• Operon: licB-licC-licA-licH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by oligomeric ß-glucosides (LicR)PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Dirk Albrecht, Rabea Sietmann, Frieder Schauer, Michael Hecker, Haike Antelmann
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis.
Environ Microbiol: 2006, 8(8);1408-27
[PubMed:16872404] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)

S Tobisch, J Stülke, M Hecker
Regulation of the lic operon of Bacillus subtilis and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis.
J Bacteriol: 1999, 181(16);4995-5003
[PubMed:10438772] [WorldCat.org] [DOI] (P p)

S Tobisch, P Glaser, S Krüger, M Hecker
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.
J Bacteriol: 1997, 179(2);496-506
[PubMed:8990303] [WorldCat.org] [DOI] (P p)