GapB

• Description: glyceraldehyde-3-phosphate dehydrogenase catalyses the syntheseis of 1,3 Bisphosphoglycerat, phosphorylation of ADP and reduction of NAD
  
  

Gene name	gapB
Synonyms	ppc
Essential	no
Product	glyceraldehyde-3-phosphate dehydrogenase2
Function	syntheseis of 1,3 Bisphosphoglycerat
MW, pI	37,3 kDa, 6.47
Gene length, protein length	1020 bp, 340 amino acids
Immediate neighbours	ytcD, speD
Gene sequence (+200bp)	Protein sequence
Genetic context File:GenE context.gif

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

• Protein family: glyceraldehyde-3-phosphate dehydrogenase family

• Paralogous protein(s): GapA

Extended information on the protein

• Kinetic information:

• Domains:
  • Nucleotid binding Domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding Domain (151-153) & (210-211)

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm

Database entries

• Structure:

• Swiss prot entry: [3]

• KEGG entry: [4]

• E.C. number: [5]

Expression and regulation

• Operon:

• Sigma factor: SigA

• Regulation: repressed (70-times) by Glc, repressor CcpN, neg. regulated by YqzB ttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+15720552

• Regulatory mechanism: transcription repression

• Additional information:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

1. Meile JC, Wu LJ, Ehrlich SD (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6(7): 2135-46. PubMed
2. Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. PubMed
3. Tännler et al. (2008) CcpN controls central carbon fluxes in Bacillus subtilis. J. Bacteriol. 190: 6178-6187. PubMed
4. Thomaides, H. B., Davison, E. J., Burston, L., Johnson, H., Brown, D. R., Hunt, A. C., Errington, J., and Czaplewski, L. (2007) Essential bacterial functions encoded by gene pairs. J Bacteriol 189, 591-602. PubMed