YfkN

• Description: extracellular 2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
  
  

Gene name	yfkN
Synonyms
Essential	yes PubMed
Product	2',3' cyclic nucleotide phosphodiesterase, 2' (or 3') nucleotidase and 5' nucleotidase
Function	probably phosphate acquisition
MW, pI	159 kDa, 5.021
Gene length, protein length	4386 bp, 1462 aa
Immediate neighbours	yfkO, yfkM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU07840

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

phosphate metabolism, membrane proteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): YhcR

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: extracellular (signal peptide) PubMed, the transmembrane domain serves as retention signal

Database entries

• Structure: 3GVE

• UniProt: O34313

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yfkN PubMed

• Sigma factor:

• Regulation:
  • repressed by glucose (5.2-fold) (CcpA) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: