hypO

hypO
168

NADPH-flavin nitroreductase

Locus
BSU_07830
Molecular weight
25.48 kDa
Isoelectric point
5.67
Protein length
221 aaSequenceBlast
Gene length
666 bpSequenceBlast
Function
protection against NaOCl stress
Product
NADPH-flavin nitroreductase
Essential
no
Synonyms
hypO, yfkO
Outlinks
BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0778 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
854,412  855,077
Phenotypes of a mutant
a ''hypO ohrA mutant is more sensitive to NaOCl stress than ohrA'' single mutant PubMed
The protein
Catalyzed reaction/ biological activity
reduction of 2,6-dinitrotoluene PubMed
Protein family
nitroreductase family (according to UniProt)
Cofactors
FMN PubMed (according to UniProt)
NADP PubMed
Structure
8QYG (PDB) (from Bacillus tequilensis, 97.8% identity) PubMed
O34475 (AlphaFold)
Expression and Regulation
Operons
Genes
hypO
Description
PubMed
Regulation
induced under disulfide stress conditions (diamide, NaOCl) (HypR) PubMed
Regulatory mechanism
HypR: activation, PubMed, in hypR regulon
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
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hypO

2025-03-14 18:49:16

ghost

79

034a13f55aabffc74aab6a4007bc149d48472e98

FAFD45B82DC9EACBD031DA4012B8352F8C8E57C3

Biological materials
Mutant
MGNA-C366 (yfkO::erm), available at the NBRP B. subtilis, Japan
BKE07830 (hypO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAACACCTCTCCTT,  downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
BKK07830 (hypO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAAAACACCTCTCCTT,  downstream forward: _UP4_TAAAAGAAGAAAGCTGCTCG
Labs working on this gene/protein
Haike Antelmann, University of Greifswald, Germany
References
Original Publications
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9DF5999568760E016ECA988385A1FA61A257D486

Page visits: 2962

Time of last update: 2025-04-02 02:30:57

Author of last update: Jstuelk