AccC

• Description: acetyl-CoA carboxylase (biotin carboxylase subunit)
  
  

Gene name	accC
Synonyms	yqhX
Essential	yes PubMed
Product	acetyl-CoA carboxylase (biotin carboxylase subunit)
Function	production of malonyl-CoA, the substrate for fatty acid biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	49 kDa, 4.72
Gene length, protein length	1350 bp, 450 aa
Immediate neighbours	yqhY, accB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU24340

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + biotin-carboxyl-carrier protein + CO₂ = ADP + phosphate + carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): YotE

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: (AccA-AccD)-AccB-AccC (acetyl-CoA carboxylase complex)

• Localization:

Database entries

• Structure: 1BNC (the protein from E. coli) PubMed

• UniProt: P49787

• KEGG entry: [3]

• E.C. number: 6.3.4.14, 6.4.1.2

Expression and regulation

• Operon: accB-accC-yqhY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Stephen W White, Jie Zheng, Yong-Mei Zhang, Rock
The structural biology of type II fatty acid biosynthesis.
Annu Rev Biochem: 2005, 74;791-831
[PubMed:15952903] [WorldCat.org] [DOI] (P p)

Original Publications

P Marini, S J Li, D Gardiol, J E Cronan, D de Mendoza
The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis.
J Bacteriol: 1995, 177(23);7003-6
[PubMed:7592499] [WorldCat.org] [DOI] (P p)

G L Waldrop, I Rayment, H M Holden
Three-dimensional structure of the biotin carboxylase subunit of acetyl-CoA carboxylase.
Biochemistry: 1994, 33(34);10249-56
[PubMed:7915138] [WorldCat.org] [DOI] (P p)