PucH

• Description: allantoinase
  
  

Gene name	pucH
Synonyms	yunH
Essential	no
Product	allantoinase
Function	purine utilization
Metabolic function and regulation of this protein in SubtiPathways: Purine catabolism, Nucleotides (regulation)
MW, pI	48 kDa, 5.062
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours	yunG, pucR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU32410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-allantoin + H₂O = allantoate (according to Swiss-Prot)

• Protein family: Allantoinase subfamily (according to Swiss-Prot)

• Paralogous protein(s): PyrC

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3HM7 (from Bacillus halodurans, 44% identity, 65% similarity)

• UniProt: O32137

• KEGG entry: [3]

• E.C. number: 3.5.2.5

Expression and regulation

• Operon: pucH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed

• Regulatory mechanism:
  • PucR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: