CtsR

• Description: transcription repressor of class III heat shock genes (clpC operon, clpE, clpP)
  
  

Gene name	ctsR
Synonyms	yacG
Essential	no
Product	transcription repressor
Function	regulation of protein degradation
Regulatory function and regulation of this protein in SubtiPathways: Stress
MW, pI	17 kDa, 9.261
Gene length, protein length	462 bp, 154 aa
Immediate neighbours	rrnW-5S, mcsA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: ctsR family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation of a tyrosine residue by McsB PubMed

• Cofactor(s):

• Effectors of protein activity: probably activated by dephosphorylation by McsA and inactivated by phosphorylation by McsB PubMed, regulated proteolysis by ClpP/ClpC PubMed, PubMed, PubMed

• Interactions: McsA-MscB-CtsR

• Localization:

Database entries

• Structure:

• Swiss prot entry: P37568

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ctsR-mcsA-mcsB-clpC-radA-disA PubMed

• Sigma factor: SigA PubMed, SigB PubMed1 PubMed2

• Regulation: induced by stress (SigB) PubMed, induced by heat (CtsR) PubMed
  • repressed at low temperatures (CtsR) PubMed

• Regulatory mechanism: CtsR: transcription repression PubMed
  • CtsR: transcription repression PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, M Hecker
The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes.
J Bacteriol: 1998, 180(24);6681-8
[PubMed:9852015] [WorldCat.org] [DOI] (P p)

1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
2. Krüger E, Hecker M. (1998) The first gene of the Bacillus subtilis clpC operon, ctsR, encodes a negative regulator of its own operon and other class III heat shock genes. J Bacteriol. 180(24): 6681-8.. PubMed