CggR

• Description: repressor of the glycolytic gapA operon
  
  

Gene name	cggR
Synonyms	yvbQ
Essential	no
Product	central glycolytic genes regulator
Function	transcriptional regulator
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	37,2 kDa,5.68
Gene length, protein length	1020 bp, 340 amino acids
Immediate neighbours	araE, gapA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU33950

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: transcription repression of the glycolytic gapA operon

• Protein family: sorC transcriptional regulatory family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:
  • DNA binding domain (H-T-H motif) (37–56)

• Modification:

• Cofactor(s):

• Effectors of protein activity: fructose 1.6-bisphosphate PubMed and dihydroxyacetone phosphate, glucose-6-phosphate and fructose-6-phosphate PubMed act as inducer and result in release of CggR from the DNA

• Interactions:

• Localization:

Database entries

• Structure: 2OKG ( effector binding domain), 3BXH (in complex with fructose-6-phosphate), complex with Fructose-6-Phosphate NCBI, effector binding domain NCBI

• Swiss prot entry: O32253

• KEGG entry: [3]

Expression and regulation

• Operon:
  • cggR-gapA-pgk-tpiA-pgm-eno
  • cggR-gapA

The primary mRNAs of the operon are highly unstable. The primary mRNA is subject to processing at the very end of the cggR open reading frame. This results in stable mature gapA and gapA-pgk-tpiA-pgm-eno mRNAs. The processing event requires the Rny protein.

• Sigma factor: SigA

• Regulation: expression activated by glucose (77 fold) PubMed, CggR represses the operon in the absence of glycolytic sugars PubMed

• Regulatory mechanism: repression

• Database entries: DBTBS

• Additional information:

Biological materials

• Mutant: GP311 (in frame deletion), available in Stülke lab

• Expression vector: pGP705 (N-terminal His-tag, in pWH844), available in Stülke lab

• lacZ fusion: pGP504 (in pAC7), pGP509 (in pAC6), available in Stülke lab

• GFP fusion:

• Antibody: available in Stülke lab

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France