Difference between revisions of "CysK"
| Line 35: | Line 35: | ||
=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU00730 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 82: | Line 82: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37887 P37887] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37887 P37887] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU00730] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.5.1.47 2.5.1.47] | * '''E.C. number:''' [http://www.expasy.org/enzyme/2.5.1.47 2.5.1.47] | ||
Revision as of 09:22, 3 June 2009
- Description: trigger enzyme: cysteine synthetase A and control of CymR activity
| Gene name | cysK |
| Synonyms | |
| Essential | no |
| Product | cysteine synthetase A |
| Function | biosynthesis of cysteine, control of CymR activity |
| MW, pI | 32 kDa, 5.492 |
| Gene length, protein length | 924 bp, 308 aa |
| Immediate neighbours | yacD, pabB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU00730
Phenotypes of a mutant
constitutive expression of the cysH-cysP-sat-cysC-ylnD-ylnE-ylnF operon, auxotrophic for cysteine
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: O(3)-acetyl-L-serine + H2S = L-cysteine + acetate (according to Swiss-Prot)
- Protein family: cysteine synthase/cystathionine beta-synthase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions: CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed
- Localization:
Database entries
- Structure:
- Swiss prot entry: P37887
- KEGG entry: [3]
- E.C. number: 2.5.1.47
Additional information
Expression and regulation
- Operon: cysK
- Regulatory mechanism: CymR: transcription repression
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Isabelle Martin-Verstraete, Institute Pasteur, Paris, France
Your additional remarks
References
- Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
- Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis.J. Biol. Chem. 283: 35551-35560. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
