Difference between revisions of "Sandbox"

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* '''Description:''' Pro-SigE protease <br/><br/>
+
* '''Description:''' RNA polymerase sporulation mother cell-specific (early) [[sigma factor]] SigE <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''spoIIGA''
+
|''sigE''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIGB ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || Pro-SigE protease
+
|style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sporulation mother cell-specific (early) [[sigma factor]] SigE
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || maturation of SigE
+
|style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (early mother cell)
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 8.552  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 9.212  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 927 bp, 309 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 717 bp, 239 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bpr]]'', ''[[sigE]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIGA]]'', ''[[sigG]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13405&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13406&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:spoIIGA_context.gif]]
+
|-
 +
|-
 +
|colspan="2" | '''Genetic context''' <br/> [[Image:sigE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIGA-sigE-sigG.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spoIIGA-sigE-sigG.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10234]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10235]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 54:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' RNA polymerase [[sigma factor]], active in the mother cell
  
 
* '''Protein family:'''
 
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 +
 +
=== Genes controlled by SigE ===
 +
 +
''[[dacB]], [[pbpI]], [[spoVD]]'', ''[[phoB]]-[[ydhF]]'', ''[[asnO]]'', ''[[bdbD]]-[[bdbC]]'', ''[[bofA]]'', ''[[cotE]]'', ''[[ctaE]]'', ''[[yaaH]]'', ''[[ybaK]]-[[cwlD]]''
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 70: Line 76:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[Processing]],  
+
* '''Interactions:''' [[SpoIIGA]]-[[SigE]],  
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 80: Line 86:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15310]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU15320]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''

Revision as of 00:02, 28 April 2009

  • Description: RNA polymerase sporulation mother cell-specific (early) sigma factor SigE

Gene name sigE
Synonyms spoIIGB
Essential no
Product RNA polymerase sporulation mother cell-specific (early) sigma factor SigE
Function transcription of sporulation genes (early mother cell)
MW, pI 27 kDa, 9.212
Gene length, protein length 717 bp, 239 aa
Immediate neighbours spoIIGA, sigG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: RNA polymerase sigma factor, active in the mother cell
  • Protein family:
  • Paralogous protein(s):

Genes controlled by SigE

dacB, pbpI, spoVD, phoB-ydhF, asnO, bdbD-bdbC, bofA, cotE, ctaE, yaaH, ybaK-cwlD

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed early during sporulation (Spo0A)
  • Regulatory mechanism: activated by Spo0A, repression by SinR
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed