Difference between revisions of "Sandbox"

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* '''Description:''' anti-RsbT, part of the stressosome <br/><br/>
+
* '''Description:''' PP2C activator, protein serine kinase, phosphorylates RsbS, part of the stressosome <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''rsbS''
+
|''rsbT''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycxS ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycxT ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || anti-RsbT
+
|style="background:#ABCDEF;" align="center"| '''Product''' || PP2C activator, protein serine kinase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of SigB activity
 
|style="background:#ABCDEF;" align="center"|'''Function''' || control of SigB activity
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 13 kDa, 4.14  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 6.587  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 363 bp, 121 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 399 bp, 133 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsbR]]'', ''[[rsbT]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rsbS]]'', ''[[rsbU]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12275&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12276&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:rsbS_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:rsbT_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 43: Line 43:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsbRSTUVW-sigB-rsbX.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsbRSTUVW-sigB-rsbX.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11091]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11092]
  
 
=== Additional information===
 
=== Additional information===
Line 64: Line 64:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
* '''Modification:''' phosphorylation on Ser-59 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
+
* '''Modification:'''
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
Line 70: Line 70:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[RsbS]]-[[RsbU]],  
+
* '''Interactions:''' [[RsbS]]-[[RsbT]],  
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:'''[http://www.uniprot.org/uniprot/P42410]
+
* '''Swiss prot entry:'''[http://www.uniprot.org/uniprot/P42411]
  
 
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU04690]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU04680]
 
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 119: Line 118:
 
=References=
 
=References=
  
# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model  bacterium ''Bacillus subtilis''. Mol. Cell. Proteomics 6: 697-707  [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 23:49, 27 April 2009

  • Description: PP2C activator, protein serine kinase, phosphorylates RsbS, part of the stressosome

Gene name rsbT
Synonyms ycxT
Essential no
Product PP2C activator, protein serine kinase
Function control of SigB activity
MW, pI 14 kDa, 6.587
Gene length, protein length 399 bp, 133 aa
Immediate neighbours rsbS, rsbU
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RsbT context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:[3]
  • KEGG entry: [4]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed