Difference between revisions of "Sandbox"

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* '''Description:''' L-aspartase <br/><br/>
+
* '''Description:''' L-asparaginase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''ansB''
+
|''ansA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || L-aspartase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || L-asparaginase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || aspartate degradation
+
|style="background:#ABCDEF;" align="center"|'''Function''' || asparagine degradation
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52 kDa, 5.69  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 4.455  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1425 bp, 475 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 987 bp, 329 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[mleN]]'', ''[[ansA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ansB]]'', ''[[ansR]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ansB_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ansA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ansB_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ansA_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;color:#FF0000" align="center" | '''Caution: The sequence for this gene in SubtiList contains errors
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ansA_context.gif]]
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:ansB_context.gif]]
 
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 46: Line 44:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ansAB.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ansAB.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10301]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10300]
  
 
=== Additional information===
 
=== Additional information===
Line 57: Line 55:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' Aspartase subfamily
+
* '''Protein family:''' asparaginase 1 family
  
* '''Paralogous protein(s):''' [[CitG]]
+
* '''Paralogous protein(s):''' [[AnsZ]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 83: Line 81:
 
* '''Swiss prot entry:'''
 
* '''Swiss prot entry:'''
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23570]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23580]
  
* '''E.C. number:''' 4.3.1.1
+
* '''E.C. number:''' 3.5.1.1
  
 
=== Additional information===
 
=== Additional information===
Line 120: Line 118:
  
 
=References=
 
=References=
 
  
 
# Sun DX, Setlow P. (1991) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. ''J Bacteriol. '' '''Jun;173(12):''' 3831-45. [http://www.ncbi.nlm.nih.gov/sites/entrez/1711029 PubMed]
 
# Sun DX, Setlow P. (1991) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. ''J Bacteriol. '' '''Jun;173(12):''' 3831-45. [http://www.ncbi.nlm.nih.gov/sites/entrez/1711029 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 05:24, 12 April 2009

  • Description: L-asparaginase

Gene name ansA
Synonyms
Essential no
Product L-asparaginase
Function asparagine degradation
MW, pI 36 kDa, 4.455
Gene length, protein length 987 bp, 329 aa
Immediate neighbours ansB, ansR
Gene sequence (+200bp) Protein sequence
Genetic context
AnsA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asparaginase 1 family
  • Paralogous protein(s): AnsZ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number: 3.5.1.1

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Sun DX, Setlow P. (1991) Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. J Bacteriol. Jun;173(12): 3831-45. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed