Difference between revisions of "Sandbox"
| Line 1: | Line 1: | ||
| − | * '''Description:''' | + | * '''Description:''' trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
| − | |'' | + | |''sacP'' |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipa-49d '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || trigger enzyme: sucrose-specific |
| + | phosphotransferase system, EIIBC component | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || sucrose uptake and phosphorylation, control of SacT activity |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49 kDa, 7.026 |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1383 bp, 461 aa |
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sacA]]'', ''[[ywcJ]]'' |
|- | |- | ||
| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ | + | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacP_nucleotide.txt Gene sequence (+200bp) ]''' |
| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ | + | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/sacP_protein.txt Protein sequence]''' |
|- | |- | ||
| − | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:sacP_context.gif]] |
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
| Line 30: | Line 31: | ||
__TOC__ | __TOC__ | ||
| − | <br/><br/> | + | <br/><br/><br/><br/> |
=The gene= | =The gene= | ||
| Line 42: | Line 43: | ||
=== Database entries === | === Database entries === | ||
| − | * '''DBTBS entry:''' | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sacPA-ywdA.html] |
| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10595] |
=== Additional information=== | === Additional information=== | ||
| Line 81: | Line 82: | ||
* '''Swiss prot entry:''' | * '''Swiss prot entry:''' | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38050] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
| Line 89: | Line 90: | ||
=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' ''[[ | + | * '''Operon:''' ''[[sacP]]-[[sacA]]-[[ywdA]]'' |
* '''Sigma factor:''' [[SigA]] | * '''Sigma factor:''' [[SigA]] | ||
| − | * '''Regulation:''' repressed by glucose ([[CcpA]]) | + | * '''Regulation:''' repressed by glucose ([[CcpA]]), repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] |
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression | ||
| Line 120: | Line 121: | ||
# Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''. Microbiology '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] | # Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of ''Bacillus subtilis''. Microbiology '''145:''' 3419-3429 [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed] | ||
| − | + | # Mäder et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] | |
| + | # Fouet, A., Arnaud, M., Klier, A., and G. Rapoport. (1987) ''Bacillus subtilis'' sucrose-specific enzyme II of the phosphotransferase system: Expression in Escherichia coli and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci USA 84: 8773-8777. [http://www.ncbi.nlm.nih.gov/sites/entrez/3122206 PubMed] | ||
| + | # Débarbouillé, M., Arnaud, M., Fouet, A., Klier, A., and Rapoport, G. (1990) The sacT gene regulating the ''sacPA'' operon in ''Bacillus subtilis'' shares strong homology with transcriptional antiterminators. J Bacteriol 172: 3966-3973. [http://www.ncbi.nlm.nih.gov/sites/entrez/2163394 PubMed] | ||
| + | # Arnaud, M., Débarbouillé, M., Rapoport, G., Saier, M. H., and Reizer, J. (1996) In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of ''Bacillus subtilis''. J Biol Chem 271: 18966-18972. [http://www.ncbi.nlm.nih.gov/sites/entrez/8702561 PubMed] | ||
| + | # Sutrina, S. L., Reddy, P., Saier, M. H., Jr & Reizer, J. (1990). The glucose permease of ''Bacillus subtilis'' is a single polypeptide chain that functions to energize the sucrose permease. J Biol Chem 265, 18581-18589. [http://www.ncbi.nlm.nih.gov/sites/entrez/2120236 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | ||
Revision as of 11:57, 6 April 2009
- Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component
| Gene name | sacP |
| Synonyms | ipa-49d |
| Essential | no |
| Product | trigger enzyme: sucrose-specific
phosphotransferase system, EIIBC component |
| Function | sucrose uptake and phosphorylation, control of SacT activity |
| MW, pI | 49 kDa, 7.026 |
| Gene length, protein length | 1383 bp, 461 aa |
| Immediate neighbours | sacA, ywcJ |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: PTS permease, sucrose permease (Scr) family PubMed
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: SigA
- Regulatory mechanism: CcpA: transcription repression
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
- Mäder et al. (2002) Transcriptome and Proteome Analysis of Bacillus subtilis Gene Expression Modulated by Amino Acid Availability. J. Bacteriol 184: 1844288-4295 PubMed
- Fouet, A., Arnaud, M., Klier, A., and G. Rapoport. (1987) Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: Expression in Escherichia coli and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci USA 84: 8773-8777. PubMed
- Débarbouillé, M., Arnaud, M., Fouet, A., Klier, A., and Rapoport, G. (1990) The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators. J Bacteriol 172: 3966-3973. PubMed
- Arnaud, M., Débarbouillé, M., Rapoport, G., Saier, M. H., and Reizer, J. (1996) In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis. J Biol Chem 271: 18966-18972. PubMed
- Sutrina, S. L., Reddy, P., Saier, M. H., Jr & Reizer, J. (1990). The glucose permease of Bacillus subtilis is a single polypeptide chain that functions to energize the sucrose permease. J Biol Chem 265, 18581-18589. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
