Difference between revisions of "Sandbox"

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* '''Description:''' write here <br/><br/>
+
* '''Description:''' two-component sensor kinase involved in phosphate regulation <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''desK ''
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|''phoR''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yocF''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' ||  
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor histidine kinase
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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|style="background:#ABCDEF;" align="center"|'''Function''' || involved in phosphate regulation
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.428  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 64 kDa, 5.957  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1110 bp, 370 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1737 bp, 579 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[des]]'', ''[[desR]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[polA]]'', ''[[phoP]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yocF_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/phoR_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/yocF_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/phoR_protein.txt Protein sequence]'''
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:yocF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:phoR_context.gif]]
 
|-
 
|-
 
|}
 
|}
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=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yocFG.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoPR.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13519]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10364]
  
 
=== Additional information===
 
=== Additional information===
Line 64: Line 64:
 
* '''Domains:'''  
 
* '''Domains:'''  
  
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* '''Modification:'''
+
* '''Modification:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
  
 
* '''Cofactor(s):'''
 
* '''Cofactor(s):'''
  
−
* '''Effectors of protein activity:'''
+
* '''Effectors of protein activity:'''  
  
−
* '''Interactions:'''
+
* '''Interactions:''' [[PhoP]]-[[PhoR]]
  
 
* '''Localization:'''
 
* '''Localization:'''
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''' ''[[phoP]]-[[phoR]]''
  
−
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]]
  
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* '''Regulation:'''  
+
* '''Regulation:''' induced by phosphate limitation ([[PhoP]]), carbon catabolite repression ([[CcpA]])
  
−
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' [[PhoP]]: transcription activation, [[CcpA]]: transcription repression
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 16:56, 9 March 2009

  • Description: two-component sensor kinase involved in phosphate regulation

Gene name phoR
Synonyms
Essential no
Product two-component sensor histidine kinase
Function involved in phosphate regulation
MW, pI 64 kDa, 5.957
Gene length, protein length 1737 bp, 579 aa
Immediate neighbours polA, phoP
Gene sequence (+200bp) Protein sequence
Genetic context
PhoR context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: two transmembrane segments, C-terminal histidine phosphotransferase domain
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by phosphate limitation (PhoP), carbon catabolite repression (CcpA)
  • Regulatory mechanism: PhoP: transcription activation, CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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