Difference between revisions of "Sandbox"

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* '''Description:''' write here <br/><br/>
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* '''Description:''' two-component sensor kinase that controls degradative enzyme synthesis and genetic competence<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''citS''
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|''degS''
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yflR ''
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|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''sacU ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor histidine kinase
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|style="background:#ABCDEF;" align="center"| '''Product''' || two-component sensor histidine kinase (EC 2.7.3.-)
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || involved in the response to the environmental Mg-citrate complex
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|style="background:#ABCDEF;" align="center"|'''Function''' || degradative enzyme and competence regulation
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 59 kDa, 6.481  
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 44 kDa, 5.988  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1626 bp, 542 aa  
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1155 bp, 385 aa  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yflS]]'', ''[[citT]]''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[degU]]'', ''[[yvyE]]''
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/citS_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/degS_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/citS_protein.txt Protein sequence]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/degS_protein.txt Protein sequence]'''
 
|-
 
|-
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|colspan="2" | '''Genetic context''' <br/> [[Image:citS_context.gif]]
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|colspan="2" | '''Genetic context''' <br/> [[Image:degS_context.gif]]
 
|-
 
|-
 
|}
 
|}
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=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citST-yflP.html]
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/degSU.html]
  
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12576]
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* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10392]
  
 
=== Additional information===
 
=== Additional information===
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:''' two transmembrane segments, C-terminal histidine phosphotransferase domain
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* '''Domains:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
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* '''Interactions:'''
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* '''Interactions:''' [[DegS]]-[[DegU]]
  
 
* '''Localization:'''
 
* '''Localization:'''
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=Expression and regulation=
 
=Expression and regulation=
  
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* '''Operon:'''  
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* '''Operon:''''' [[degS]]-[[degU]]''
  
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* '''Sigma factor:'''  
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* '''Sigma factor:''' [[SigA]]
  
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* '''Regulation:'''  
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* '''Regulation:''' induced in the presence of ammonium ([[TnrA]])
  
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* '''Regulatory mechanism:'''  
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* '''Regulatory mechanism:''' [[TnrA]]: repression, [[DegU]]: positive autoregulation
  
−
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Tarek Msadek]], Institut Pasteur, Paris, France
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 16:55, 9 March 2009

  • Description: two-component sensor kinase that controls degradative enzyme synthesis and genetic competence

Gene name degS
Synonyms sacU
Essential no
Product two-component sensor histidine kinase (EC 2.7.3.-)
Function degradative enzyme and competence regulation
MW, pI 44 kDa, 5.988
Gene length, protein length 1155 bp, 385 aa
Immediate neighbours degU, yvyE
Gene sequence (+200bp) Protein sequence
Genetic context
DegS context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced in the presence of ammonium (TnrA)
  • Regulatory mechanism: TnrA: repression, DegU: positive autoregulation
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Tarek Msadek, Institut Pasteur, Paris, France

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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