Ald

• Description: L-alanine dehydrogenase
  
  

Gene name	ald
Synonyms	spoVN
Essential	no
Product	L-alanine dehydrogenase
Function	alanine utilization
Gene expression levels in SubtiExpress: ald
Metabolic function and regulation of this protein in SubtiPathways: ald
MW, pI	39 kDa, 5.125
Gene length, protein length	1134 bp, 378 aa
Immediate neighbours	adeR, yukJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AdeR regulon

The gene

Basic information

• Locus tag: BSU31930

Phenotypes of a mutant

• sporulation defect PubMed

Database entries

• BsubCyc: BSU31930

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-alanine + H₂O + NAD⁺ = pyruvate + NH₃ + NADH (according to Swiss-Prot)

• Protein family: AlaDH/PNT family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU31930

• Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)

• UniProt: Q08352

• KEGG entry: [3]

• E.C. number: 1.4.1.1

Expression and regulation

• Operon: ald PubMed

• Expression browser: ald PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by alanine (AdeR) PubMed

• Regulatory mechanism:
  • AdeR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 15145 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 6392 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 15279 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Alexandra Lerchner, Alexander Jarasch, Arne Skerra
Engineering of alanine dehydrogenase from Bacillus subtilis for novel cofactor specificity.
Biotechnol Appl Biochem: 2016, 63(5);616-624
[PubMed:26202482] [WorldCat.org] [DOI] (I p)

Ta-Hui Lin, Guei-Tsung Wei, Chien-Chen Su, Gwo-Chyuan Shaw
AdeR, a PucR-type transcription factor, activates expression of L-alanine dehydrogenase and is required for sporulation of Bacillus subtilis.
J Bacteriol: 2012, 194(18);4995-5001
[PubMed:22797752] [WorldCat.org] [DOI] (I p)

Jessica C Wilks, Ryan D Kitko, Sarah H Cleeton, Grace E Lee, Chinagozi S Ugwu, Brian D Jones, Sandra S BonDurant, Joan L Slonczewski
Acid and base stress and transcriptomic responses in Bacillus subtilis.
Appl Environ Microbiol: 2009, 75(4);981-90
[PubMed:19114526] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219] [WorldCat.org] [DOI] (P p)

Bauke Oudega, Micheline Vandenbol, Gregory Koningstein
A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
Microbiology (Reading): 1997, 143 ( Pt 5);1489-1491
[PubMed:9168598] [WorldCat.org] [DOI] (P p)

K J Siranosian, K Ireton, A D Grossman
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
J Bacteriol: 1993, 175(21);6789-96
[PubMed:8226620] [WorldCat.org] [DOI] (P p)