LytF

• Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase, cell separase (major autolysin)
  
  

Gene name	lytF
Synonyms	cwlE, yhdD
Essential	no
Product	gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
Function	cell separation
Gene expression levels in SubtiExpress: lytF
Interactions involving this protein in SubtInteract: LytF
Metabolic function and regulation of this protein in SubtiPathways: LytF
MW, pI	51 kDa, 10.41
Gene length, protein length	1464 bp, 488 aa
Immediate neighbours	yhdC, nsrR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall degradation/ turnover

This gene is a member of the following regulons

SigD regulon, SlrR regulon

The gene

Basic information

• Locus tag: BSU09370

Phenotypes of a mutant

• cell separation defect, this is increased by a lytE mutation PubMed

Database entries

• BsubCyc: BSU09370

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nlpC/p60 family (according to Swiss-Prot)

• Paralogous protein(s): the C-terminal D,L-endopeptidase domains of LytE, LytF, CwlS, and CwlO exhibit strong sequence similarity

Extended information on the protein

• Kinetic information:

• Domains:
  • contains five N-acetylglucosamine-polymer-binding LysM domains PubMed
  • C-terminal D,L-endopeptidase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:
  • IseA-LytF (catalytic domain) PubMed

• Localization:
  • secreted (according to Swiss-Prot)
  • localizes to cell septa and poles PubMed

Database entries

• BsubCyc: BSU09370

• Structure:

• UniProt: O07532

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: lytF PubMed

• Expression browser: lytF PubMed

• Sigma factor: SigD PubMed

• Regulation:

• Regulatory mechanism:
  • SlrR (in complex with SinR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A791 ( lytF::spec), PubMed, available at BGSC
  • 1A792 ( lytF::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: