LutB

• Description: lactate catabolic enzyme
  
  

Gene name	lutB
Synonyms	yvfW
Essential	no
Product	lactate oxidase
Function	lactate utilization
Gene expression levels in SubtiExpress: lutB
Metabolic function and regulation of this protein in SubtiPathways: lutB
MW, pI	53 kDa, 5.848
Gene length, protein length	1437 bp, 479 aa
Immediate neighbours	lutC, lutA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, phosphoproteins

This gene is a member of the following regulons

FbpB regulon, FsrA regulon, LutR regulon, SinR regulon

The gene

Basic information

• Locus tag: BSU34040

Phenotypes of a mutant

no growth with lactate as the single carbon source PubMed

Database entries

• BsubCyc: BSU34040

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: oxidation of lactate to pyruvate PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-33 PubMed

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU34040

• Structure:

• UniProt: O07021

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: lutA-lutB-lutC PubMed

• Expression browser: lutB PubMed

• Sigma factor:

• Regulation:
  • repressed by SinR PubMed
  • induction by lactate PubMed
  • less expressed under conditions of extreme iron limitation (FsrA) PubMed
  • part of the iron sparing response, down-regulation in a fur mutant (Fur, FsrA, FpbB) PubMed

• Regulatory mechanism:
  • SinR: transcription repression PubMed
  • dual repression by LutR (inducer lactate) and SinR PubMed
  • FsrA: translational repression PubMed
  • FbpB: acts as RNA chaperone, increases interaction between fsrA and lutA-lutB-lutC RNAs PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 246 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 4124 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Richard Losick, Harvard Univ., Cambridge, USA homepage