MtnD

• Description: 1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
  
  

Gene name	mtnD
Synonyms	ykrZ
Essential	no
Product	1,2,-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Function	methionine salvage
Gene expression levels in SubtiExpress: mtnD
Metabolic function and regulation of this protein in SubtiPathways: mtnD
MW, pI	20 kDa, 4.408
Gene length, protein length	534 bp, 178 aa
Immediate neighbours	mtnB, ykvA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, most abundant proteins

This gene is a member of the following regulons

S-box

The gene

Basic information

• Locus tag: BSU13620

Database entries

• BsubCyc: BSU13620

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O₂ = 3-(methylthio)propanoate + formate + CO (according to Swiss-Prot)

• Protein family: acireductone dioxygenase (ARD) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU13620

• Structure:

• UniProt: O31669

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtnW-mtnX-mtnB-mtnD PubMed

• Expression browser: mtnD PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed
  • induced by methionine starvation (S-box) PubMed

• Regulatory mechanism: S-box: transcription termination/ antitermination, the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 4182 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1417 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: