Difference between revisions of "CitG"
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
| − | {{SubtiWiki category|[[carbon core metabolism]]}} | + | {{SubtiWiki category|[[carbon core metabolism]]}}, |
| + | [[most abundant proteins]] | ||
= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
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=== Additional information=== | === Additional information=== | ||
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| − | |||
| − | |||
=The protein= | =The protein= | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' | + | * '''[[Domains]]:''' |
* '''Modification:''' | * '''Modification:''' | ||
| − | * ''' | + | * '''[[Cofactors]]:''' |
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fumC_3389024_3390412_-1 citG] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fumC_3389024_3390412_-1 citG] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' [[SigA]] {{PubMed|3130545,2509422,2509423}}, [[SigH]] {{PubMed|3130545,2509422}} | + | * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3130545,2509422,2509423}}, [[SigH]] {{PubMed|3130545,2509422}} |
* '''Regulation:''' constitutive | * '''Regulation:''' constitutive | ||
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* '''Additional information:''' | * '''Additional information:''' | ||
| + | ** belongs to the 100 [[most abundant proteins]] {{PubMed|15378759}} | ||
=Biological materials = | =Biological materials = | ||
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=References= | =References= | ||
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| − | <pubmed>2509422,3923430 ,3130545 2509423 20933603</pubmed> | + | <pubmed>2509422,3923430 ,3130545 2509423 21661762 20933603 15378759</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 17:40, 5 March 2014
- Description: fumarase
| Gene name | citG |
| Synonyms | |
| Essential | no |
| Product | fumarate hydratase |
| Function | TCA cycle |
| Gene expression levels in SubtiExpress: citG | |
| Interactions involving this protein in SubtInteract: CitG | |
| Metabolic function and regulation of this protein in SubtiPathways: citG | |
| MW, pI | 50 kDa, 5.475 |
| Gene length, protein length | 1386 bp, 462 aa |
| Immediate neighbours | yuxN, yvzF |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
carbon core metabolism, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU33040
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: (S)-malate = fumarate + H2O (according to Swiss-Prot)
- Protein family: Fumarase subfamily (according to Swiss-Prot)
- Paralogous protein(s): AnsB
Extended information on the protein
- Kinetic information:
- Modification:
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: P07343
- KEGG entry: [3]
- E.C. number: 4.2.1.2
Additional information
Expression and regulation
- Regulation: constitutive
- Regulatory mechanism:
- Additional information:
- belongs to the 100 most abundant proteins PubMed
Biological materials
- Mutant:
- GP718 (spec), available in Jörg Stülke's lab
- Expression vector:
- pGP1122 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Jörg Stülke's lab)
- lacZ fusion:
- pGP387 (in pAC7), available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- Antibody:
- FLAG-tag construct:
- GP1132 (spc, based on pGP1331), available in Jörg Stülke's lab
Labs working on this gene/protein
Your additional remarks
References
