Abn2

• Description: endo-1,5-alpha-L-arabinosidase
  
  

Gene name	abn2
Synonyms	yxiA
Essential	no
Product	endo-1,5-alpha-L-arabinosidase
Function	arabinan degradation
Gene expression levels in SubtiExpress: abn2
MW, pI	52 kDa, 7.371
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	yxiB, yxzL
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context

Categories containing this gene/protein

utilization of specific carbon sources

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: glycosyl hydrolase 43 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information: With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. PubMed

• Domains:
  • N-terminal catalytic domain with a characteristic β-propeller fold and a C-terminal domain whose function is unknown PubMed

• Modification:

• Cofactors: Ca²⁺ PubMed

• Effectors of protein activity:

• Interactions:

• Localization:
  • extracellular (signal peptide) PubMed

Database entries

• Structure: 2X8F PubMed

• UniProt: P42293

• KEGG entry: [3]

• E.C. number: 3.2.1.99

Expression and regulation

• Operon: abn2 PubMed

• Sigma factor: SigA, SigH PubMed

• Regulation:
  • repressed by glucose (4.3-fold) (CcpA) PubMed
  • expression is stimulated by arabinose and pectin and repressed by glucose PubMed

• Regulatory mechanism:
  • CcpA: transcription repression, unknown PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Isabel de Sa-Nogueira, Lisboa, Portugal homepage