Difference between revisions of "PtkA"
(→Biological materials) |
(→Biological materials) |
||
Line 124: | Line 124: | ||
=Biological materials = | =Biological materials = | ||
− | * '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]; GP1520 (spc), available in [[Stülke]] lab; GP1521 epsB (aphA3) ptkA (spc) double mutant available in [[Stülke]] lab; GP1529 tkmA-ptkA::spc available in [[Stülke]] lab | + | * '''Mutant:''' KO strain created with pMUTIN-2, available from [[Ivan Mijakovic]]; GP1520 (spc), GP1544 (ermC) and GP1587 (cat) , available in [[Stülke]] lab; GP1521 epsB (aphA3) ptkA (spc) double mutant available in [[Stülke]] lab; GP1529 tkmA-ptkA::spc available in [[Stülke]] lab |
* '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]] | * '''Expression vector:''' pQE-30, N-terminally 6xHis-tagged, available from [[Ivan Mijakovic]] |
Revision as of 16:59, 27 January 2014
- Description: protein tyrosine kinase
Gene name | ptkA |
Synonyms | ywqD |
Essential | no |
Product | protein tyrosine kinase |
Function | protein phosphorylation |
Gene expression levels in SubtiExpress: ptkA | |
Interactions involving this protein in SubtInteract: PtkA | |
MW, pI | 25 kDa, 9.628 |
Gene length, protein length | 711 bp, 237 aa |
Immediate neighbours | ptpZ, tkmA |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
biofilm formation, protein modification, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU36250
Phenotypes of a mutant
- Accumulation of extra chromosome equivalents PubMed
- Defect in biofilm formation, this involves the kinase activity, but the target protein is unknown PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of Ugd, TuaD, Ssb, SsbB
- Protein family: BY-kinase, see the Bacterial Protein Tyrosine Kinase Database)
- Paralogous protein(s): EpsB
Extended information on the protein
- Kinetic information:
- Domains: single BY-kinase domain
- Modification: autophosphorylation at residues Y225, Y227 and Y228 (primary site) PubMed, dephosphorylated by PtpZ PubMed
- Cofactor(s): ATP
- Effectors of protein activity: TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation PubMed
Database entries
- Structure: 2VED (CapB, the homolog in Staphylococcus aureus)
- UniProt: P96716
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Additional information:
Biological materials
- Mutant: KO strain created with pMUTIN-2, available from Ivan Mijakovic; GP1520 (spc), GP1544 (ermC) and GP1587 (cat) , available in Stülke lab; GP1521 epsB (aphA3) ptkA (spc) double mutant available in Stülke lab; GP1529 tkmA-ptkA::spc available in Stülke lab
- Expression vector: pQE-30, N-terminally 6xHis-tagged, available from Ivan Mijakovic
- lacZ fusion: in a KO strain created with pMUTIN-2, available from Ivan Mijakovic
- GFP fusion: CFP-fusion, available from Ivan Mijakovic
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Ivan Mijakovic, Thiverval-Grignon, France
Your additional remarks
References
Reviews
Jörg Stülke
More than just activity control: phosphorylation may control all aspects of a protein's properties.
Mol Microbiol: 2010, 77(2);273-5
[PubMed:20497498]
[WorldCat.org]
[DOI]
(I p)
Original publications