Difference between revisions of "EpsA"
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− | * '''Mutant:''' | + | * '''Mutant:''' GP1517 (aphA3), available in [[Stülke]] lab |
* '''Expression vector:''' | * '''Expression vector:''' |
Revision as of 16:35, 27 January 2014
- Description: extracellular polysaccharide synthesis, putative transmembrane modulator of EpsB activity, might activate EpsB autophosphorylation and substrate phosphorylation
Gene name | epsA |
Synonyms | yveK |
Essential | no |
Product | unknown |
Function | biofilm formation |
Gene expression levels in SubtiExpress: epsA | |
Interactions involving this protein in SubtInteract: EpsA | |
Regulation of this protein in SubtiPathways: epsA | |
MW, pI | 25 kDa, 6.071 |
Gene length, protein length | 702 bp, 234 aa |
Immediate neighbours | epsB, slrR |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
protein modification, biofilm formation, membrane proteins
This gene is a member of the following regulons
AbrB regulon, RemA regulon, SinR regulon
The gene
Basic information
- Locus tag: BSU34370
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: cpsC/capA family (according to Swiss-Prot)
- Paralogous protein(s): TkmA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P71050
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
- induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
- the epsA-epsB-epsC-epsD-epsE-epsF-epsG-epsH-epsI-epsJ-epsK-epsL-epsM-epsN-epsO operon is not expressed in a ymdB mutant PubMed
- the amount of the mRNA is substantially decreased upon depletion of RNase Y (this is likely due to the increased stability of the sinR mRNA) PubMed
Biological materials
- Mutant: GP1517 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Richard Losick, Harvard Univ., Cambridge, USA homepage
Your additional remarks
References
Reviews
Original publications