Difference between revisions of "CitM"

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|style="background:#ABCDEF;" align="center"| '''Product''' || Mg2+-citrate transporter
 
|style="background:#ABCDEF;" align="center"| '''Product''' || Mg2+-citrate transporter
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || citrate uptake  
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|style="background:#ABCDEF;" align="center"|'''Function''' ||  uptake of citrate and magnesium
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07610 citM]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU07610 citM]
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[transporters/ other]]}},
 
{{SubtiWiki category|[[transporters/ other]]}},
 
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
 
{{SubtiWiki category|[[utilization of specific carbon sources]]}},
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{{SubtiWiki category|[[metal ion homeostasis (K, Na, Ca, Mg)]]}},
 
{{SubtiWiki category|[[membrane proteins]]}}
 
{{SubtiWiki category|[[membrane proteins]]}}
  
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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no growth with citrate as sole carbon source {{PubMed|10972810}}
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** no growth with citrate as sole carbon source {{PubMed|10972810}}
  
 
=== Database entries ===
 
=== Database entries ===
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=== Additional information===
 
=== Additional information===
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=The protein=
 
=The protein=
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* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
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* '''Domains:'''  
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* '''[[Domains]]:'''  
  
 
* '''Modification:'''
 
* '''Modification:'''
  
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* '''Cofactor(s):'''
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* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=citM_834383_835684_1 citM] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=citM_834383_835684_1 citM] {{PubMed|22383849}}
  
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* '''Sigma factor:''' [[SigA]] {{PubMed|10972810}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|10972810}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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=References=
 
=References=
  
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<pubmed>16842348,11891560,10972810,11053381,3127276,12375105,8892821,12533460,12670692,11029430,12427932, 22900538 </pubmed>
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<pubmed>16842348,11891560,10972810,11053381,3127276,12375105,8892821,12533460,12670692,11029430,12427932, 22900538 24415722</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:20, 16 January 2014

  • Description: Mg2+-citrate transporter

Gene name citM
Synonyms yflO
Essential no
Product Mg2+-citrate transporter
Function uptake of citrate and magnesium
Gene expression levels in SubtiExpress: citM
Metabolic function and regulation of this protein in SubtiPathways:
citM
MW, pI 45 kDa, 6.227
Gene length, protein length 1299 bp, 433 aa
Immediate neighbours yflP, yflN
Sequences Protein DNA DNA_with_flanks
Genetic context
CitM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CitM expression.png















Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, metal ion homeostasis (K, Na, Ca, Mg), membrane proteins

This gene is a member of the following regulons

CcpA regulon, CitT regulon

The gene

Basic information

  • Locus tag: BSU07610

Phenotypes of a mutant

    • no growth with citrate as sole carbon source PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: View classification (according to Swiss-Prot)
  • Paralogous protein(s): CitH

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Catherine A Wakeman, Jonathan R Goodson, Vineetha M Zacharia, Wade C Winkler
Assessment of the requirements for magnesium transporters in Bacillus subtilis.
J Bacteriol: 2014, 196(6);1206-14
[PubMed:24415722] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Guillermo D Repizo, Víctor S Blancato, Pablo D Sender, Juke Lolkema, Christian Magni
Catabolite repression of the citST two-component system in Bacillus subtilis.
FEMS Microbiol Lett: 2006, 260(2);224-31
[PubMed:16842348] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
A Crh-specific function in carbon catabolite repression in Bacillus subtilis.
FEMS Microbiol Lett: 2003, 220(2);277-80
[PubMed:12670692] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Christian Magni, Juke S Lolkema
CcpA-independent regulation of expression of the Mg2+ -citrate transporter gene citM by arginine metabolism in Bacillus subtilis.
J Bacteriol: 2003, 185(3);854-9
[PubMed:12533460] [WorldCat.org] [DOI] (P p)

Jessica B Warner, Juke S Lolkema
Growth of Bacillus subtilis on citrate and isocitrate is supported by the Mg2+-citrate transporter CitM.
Microbiology (Reading): 2002, 148(Pt 11);3405-3412
[PubMed:12427932] [WorldCat.org] [DOI] (P p)

Bastiaan P Krom, Henry Huttinga, Jessica B Warner, Juke S Lolkema
Impact of the Mg(2+)-citrate transporter CitM on heavy metal toxicity in Bacillus subtilis.
Arch Microbiol: 2002, 178(5);370-5
[PubMed:12375105] [WorldCat.org] [DOI] (P p)

H Li, A M Pajor
Functional characterization of CitM, the Mg2+-citrate transporter.
J Membr Biol: 2002, 185(1);9-16
[PubMed:11891560] [WorldCat.org] [DOI] (P p)

B P Krom, J B Warner, W N Konings, J S Lolkema
Complementary metal ion specificity of the metal-citrate transporters CitM and CitH of Bacillus subtilis.
J Bacteriol: 2000, 182(22);6374-81
[PubMed:11053381] [WorldCat.org] [DOI] (P p)

J B Warner, B P Krom, C Magni, W N Konings, J S Lolkema
Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.
J Bacteriol: 2000, 182(21);6099-105
[PubMed:11029430] [WorldCat.org] [DOI] (P p)

H Yamamoto, M Murata, J Sekiguchi
The CitST two-component system regulates the expression of the Mg-citrate transporter in Bacillus subtilis.
Mol Microbiol: 2000, 37(4);898-912
[PubMed:10972810] [WorldCat.org] [DOI] (P p)

A Boorsma, M E van der Rest, J S Lolkema, W N Konings
Secondary transporters for citrate and the Mg(2+)-citrate complex in Bacillus subtilis are homologous proteins.
J Bacteriol: 1996, 178(21);6216-22
[PubMed:8892821] [WorldCat.org] [DOI] (P p)

P Carlsson, L Hederstedt
Bacillus subtilis citM, the structural gene for dihydrolipoamide transsuccinylase: cloning and expression in Escherichia coli.
Gene: 1987, 61(2);217-24
[PubMed:3127276] [WorldCat.org] [DOI] (P p)

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