Difference between revisions of "RpoY"

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=== Additional information===
 
=== Additional information===
 
The protein is sometimes annotated as omega1 subunit of [[RNA polymerase]], however, there is no experimental evidence in support of this ("it is unrelated in sequence to ω/Rpb6 from the eubacteria, archaea and eukaryotes, unpublished data suggests it does not bind at the same site as ω/Rpb6 subunits bind their cognate RNAPs, and therefore it is unlikely to function as a true ω subunit") {{PubMed|20724389}}  
 
The protein is sometimes annotated as omega1 subunit of [[RNA polymerase]], however, there is no experimental evidence in support of this ("it is unrelated in sequence to ω/Rpb6 from the eubacteria, archaea and eukaryotes, unpublished data suggests it does not bind at the same site as ω/Rpb6 subunits bind their cognate RNAPs, and therefore it is unlikely to function as a true ω subunit") {{PubMed|20724389}}  
 
 
  
 
=The protein=
 
=The protein=
Line 102: Line 100:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''[[ykzG]]-[[rnjA]]'' {{PubMed|24187087}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykzG_1524791_1525000_-1 ykzG] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykzG_1524791_1525000_-1 ykzG] {{PubMed|22383849}}
  
* '''[[Sigma factor]]:'''  
+
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|24187087}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 113: Line 111:
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** translation of [[YkzG]] and [[RnjA]] is coupled, and this coupling is required for efficient expression of [[rnjA|RNase J1]] {{PubMed|24187087}}
  
 
=Biological materials =
 
=Biological materials =
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=References=
 
=References=
'''Additional publications:''' {{PubMed|20724389}}
+
<pubmed>24187087 20724389</pubmed>
 +
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:23, 13 November 2013

Gene name ykzG
Synonyms
Essential no
Product epsilon subunit of RNA polymerase
Function control of RNA polymerase activity
Gene expression levels in SubtiExpress: ykzG
MW, pI 8 kDa, 4.605
Gene length, protein length 207 bp, 69 aa
Immediate neighbours rnjA, ykrA
Sequences Protein DNA DNA_with_flanks
Genetic context
YkzG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YkzG expression.png















Categories containing this gene/protein

transcription

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU14540

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein is sometimes annotated as omega1 subunit of RNA polymerase, however, there is no experimental evidence in support of this ("it is unrelated in sequence to ω/Rpb6 from the eubacteria, archaea and eukaryotes, unpublished data suggests it does not bind at the same site as ω/Rpb6 subunits bind their cognate RNAPs, and therefore it is unlikely to function as a true ω subunit") PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: UPF0356 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • translation of YkzG and RnjA is coupled, and this coupling is required for efficient expression of RNase J1 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP420 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References