Difference between revisions of "YqhY"
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* '''Mutant:''' | * '''Mutant:''' | ||
| + | ** GP1468 (''yqhY''::''erm''), available in [[Jörg Stülke]]'s lab | ||
* '''Expression vector:''' | * '''Expression vector:''' | ||
| + | ** GP1474 (chromosomal ''yqhY''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [[SPINE]], available in [[Jörg Stülke]]'s lab''' | ||
| + | ** pGP1322 (N-terminal Strep-tag, purification from ''E. coli'', in [[pGP172]]), available in [[Jörg Stülke]]'s lab | ||
| + | ** pGP1325 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab | ||
* '''lacZ fusion:''' | * '''lacZ fusion:''' | ||
* '''GFP fusion:''' | * '''GFP fusion:''' | ||
| + | ** pBP43-Gfp-yqhY, available in [[Jörg Stülke]]'s lab | ||
| − | * '''two-hybrid system:''' | + | * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab |
| + | |||
| + | * '''FLAG-tag construct:''' | ||
| + | ** GP1476 (''spc'' ''aphA''3, based on [[pGP1331]]), available in [[Jörg Stülke]]'s lab | ||
* '''Antibody:''' | * '''Antibody:''' | ||
Revision as of 09:00, 19 September 2013
- Description: unknown
| Gene name | yqhY |
| Synonyms | |
| Essential | yes PubMed |
| Product | unknown |
| Function | unknown |
| Gene expression levels in SubtiExpress: yqhY | |
| Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis | |
| MW, pI | 14 kDa, 4.414 |
| Gene length, protein length | 405 bp, 135 aa |
| Immediate neighbours | nusB, accC |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
proteins of unknown function, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU24330
Phenotypes of a mutant
essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: asp23 family (according to Swiss-Prot)
- Paralogous protein(s): YloU
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- Structure:
- UniProt: P54519
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1468 (yqhY::erm), available in Jörg Stülke's lab
- Expression vector:
- GP1474 (chromosomal yqhY-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
- pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
- pGP1325 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
- lacZ fusion:
- GFP fusion:
- pBP43-Gfp-yqhY, available in Jörg Stülke's lab
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
- FLAG-tag construct:
- GP1476 (spc aphA3, based on pGP1331), available in Jörg Stülke's lab
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
