Difference between revisions of "YkuI"
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| − | * '''Description:''' | + | * '''Description:''' c-di-GMP binding protein <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"| '''Product''' || | + | |style="background:#ABCDEF;" align="center"| '''Product''' || c-di-GMP binding protein |
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown | |style="background:#ABCDEF;" align="center"|'''Function''' || unknown | ||
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=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' |
* '''Protein family:''' | * '''Protein family:''' | ||
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* '''Kinetic information:''' | * '''Kinetic information:''' | ||
| − | * '''Domains:''' EAL domain (aa 1 ... 245 | + | * '''Domains:''' EAL domain (aa 1 ... 245), PAS-like domain (aa 300 ... 400) [http://www.ncbi.nlm.nih.gov/sites/entrez/19244251 PubMed] |
* '''Modification:''' | * '''Modification:''' | ||
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2BAS 2BAS] | + | * '''Structure:''' |
| + | ** [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2BAS 2BAS] | ||
| + | ** in complex with c-di-GMP [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2W27 2W27] [http://www.ncbi.nlm.nih.gov/sites/entrez/19244251 PubMed] | ||
* '''UniProt:''' [http://www.uniprot.org/uniprot/O35014 O35014] | * '''UniProt:''' [http://www.uniprot.org/uniprot/O35014 O35014] | ||
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykuI_1482248_1483471_1 ykuI] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ykuI_1482248_1483471_1 ykuI] {{PubMed|22383849}} | ||
| − | * '''Sigma factor:''' | + | * '''[[Sigma factor]]:''' |
* '''Regulation:''' | * '''Regulation:''' | ||
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=References= | =References= | ||
| − | <pubmed> 19244251 22821967</pubmed> | + | <pubmed> 23893111 19244251 22821967</pubmed> |
[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 12:05, 10 August 2013
- Description: c-di-GMP binding protein
| Gene name | ykuI |
| Synonyms | |
| Essential | no |
| Product | c-di-GMP binding protein |
| Function | unknown |
| Gene expression levels in SubtiExpress: ykuI | |
| MW, pI | 47 kDa, 4.462 |
| Gene length, protein length | 1221 bp, 407 aa |
| Immediate neighbours | ykuH, fsrA |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
metabolism of signalling nucleotides
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU14090
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains: EAL domain (aa 1 ... 245), PAS-like domain (aa 300 ... 400) PubMed
- Modification:
- Cofactor(s):
- Effectors of protein activity:
Database entries
- UniProt: O35014
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- GP1324 (tet), available in Jörg Stülke's lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Xiaohui Gao, Sampriti Mukherjee, Paige M Matthews, Loubna A Hammad, Daniel B Kearns, Charles E Dann
Functional characterization of core components of the Bacillus subtilis cyclic-di-GMP signaling pathway.
J Bacteriol: 2013, 195(21);4782-92
[PubMed:23893111]
[WorldCat.org]
[DOI]
(I p)
Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J Bacteriol: 2012, 194(18);5080-90
[PubMed:22821967]
[WorldCat.org]
[DOI]
(I p)
George Minasov, Sivaraman Padavattan, Ludmilla Shuvalova, Joseph S Brunzelle, Darcie J Miller, Arnaud Baslé, Claudia Massa, Frank R Collart, Tilman Schirmer, Wayne F Anderson
Crystal structures of YkuI and its complex with second messenger cyclic Di-GMP suggest catalytic mechanism of phosphodiester bond cleavage by EAL domains.
J Biol Chem: 2009, 284(19);13174-84
[PubMed:19244251]
[WorldCat.org]
[DOI]
(P p)
