Difference between revisions of "NrgA"

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|style="background:#ABCDEF;" align="center"|'''Function''' || ammonium uptake
 
|style="background:#ABCDEF;" align="center"|'''Function''' || ammonium uptake
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU36510 nrgA]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36510 nrgA]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrgA NrgA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NrgA NrgA]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywoB]]'', ''[[nrgB]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywoB]]'', ''[[nrgB]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15668&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU36510 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU36510 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU36510 Advanced_DNA]
 
|-
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:nrgA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:nrgA_context.gif]]

Revision as of 14:03, 13 May 2013

  • Description: ammonium transporter, required at low ammonium concentration

Gene name nrgA
Synonyms amtB
Essential no
Product ammonium transporter
Function ammonium uptake
Gene expression levels in SubtiExpress: nrgA
Interactions involving this protein in SubtInteract: NrgA
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 42 kDa, 5.727
Gene length, protein length 1212 bp, 404 aa
Immediate neighbours ywoB, nrgB
Sequences Protein DNA Advanced_DNA
Genetic context
NrgA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NrgA expression.png




























Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids, glutamate metabolism, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by ammonium limitation
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP739 (cat), GP255 (nrgAB, cat), both available in the Stülke lab
  • Expression vector:
  • lacZ fusion: pGP168 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References

Reviews

Arnaud Javelle, Domenico Lupo, Xiao-Dan Li, Mike Merrick, Mohamed Chami, Pierre Ripoche, Fritz K Winkler
Structural and mechanistic aspects of Amt/Rh proteins.
J Struct Biol: 2007, 158(3);472-81
[PubMed:17368911] [WorldCat.org] [DOI] (P p)

G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet: 2000, 16(1);11-4
[PubMed:10637624] [WorldCat.org] [DOI] (P p)

Original Publications