Difference between revisions of "Ald"
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|style="background:#ABCDEF;" align="center"|'''Function''' || alanine utilization | |style="background:#ABCDEF;" align="center"|'''Function''' || alanine utilization | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU31930 ald] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ala_gly_ser.html Ala, Gly, Ser]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ala_gly_ser.html Ala, Gly, Ser]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[adeR]]'', ''[[yukJ]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[adeR]]'', ''[[yukJ]]'' | ||
|- | |- | ||
| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU31930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU31930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU31930 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:ald_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:ald_context.gif]] | ||
Revision as of 13:45, 13 May 2013
- Description: L-alanine dehydrogenase
| Gene name | ald |
| Synonyms | spoVN |
| Essential | no |
| Product | L-alanine dehydrogenase |
| Function | alanine utilization |
| Gene expression levels in SubtiExpress: ald | |
| Metabolic function and regulation of this protein in SubtiPathways: Ala, Gly, Ser | |
| MW, pI | 39 kDa, 5.125 |
| Gene length, protein length | 1134 bp, 378 aa |
| Immediate neighbours | adeR, yukJ |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU31930
Phenotypes of a mutant
- sporulation defect PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH (according to Swiss-Prot)
- Protein family: AlaDH/PNT family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure: 2EEZ (from Thermus thermophilus hb8 mutant, 55% identity, 66% similarity)
- UniProt: Q08352
- KEGG entry: [3]
- E.C. number: 1.4.1.1
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Additional publications: PubMed
Jessica C Wilks, Ryan D Kitko, Sarah H Cleeton, Grace E Lee, Chinagozi S Ugwu, Brian D Jones, Sandra S BonDurant, Joan L Slonczewski
Acid and base stress and transcriptomic responses in Bacillus subtilis.
Appl Environ Microbiol: 2009, 75(4);981-90
[PubMed:19114526]
[WorldCat.org]
[DOI]
(I p)
Le Thi Tam, Christine Eymann, Haike Antelmann, Dirk Albrecht, Michael Hecker
Global gene expression profiling of Bacillus subtilis in response to ammonium and tryptophan starvation as revealed by transcriptome and proteome analysis.
J Mol Microbiol Biotechnol: 2007, 12(1-2);121-30
[PubMed:17183219]
[WorldCat.org]
[DOI]
(P p)
Bauke Oudega, Micheline Vandenbol, Gregory Koningstein
A 12 kb nucleotide sequence containing the alanine dehydrogenase gene at 279 degrees on the Bacillus subtilis chromosome.
Microbiology (Reading): 1997, 143 ( Pt 5);1489-1491
[PubMed:9168598]
[WorldCat.org]
[DOI]
(P p)
K J Siranosian, K Ireton, A D Grossman
Alanine dehydrogenase (ald) is required for normal sporulation in Bacillus subtilis.
J Bacteriol: 1993, 175(21);6789-96
[PubMed:8226620]
[WorldCat.org]
[DOI]
(P p)
