Difference between revisions of "DnaG"

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|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]]
 
|style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA replication]]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU25210 dnaG]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU25210 dnaG]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DnaG DnaG]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/DnaG DnaG]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigA]]'', ''[[antE]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sigA]]'', ''[[antE]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14451&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU25210 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU25210 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU25210 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:dnaG_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:dnaG_context.gif]]

Revision as of 13:19, 13 May 2013

  • Description: DNA primase, part of the replisome

Gene name dnaG
Synonyms dnaE
Essential yes PubMed
Product DNA primase
Function DNA replication
Gene expression levels in SubtiExpress: dnaG
Interactions involving this protein in SubtInteract: DnaG
MW, pI 68 kDa, 6.706
Gene length, protein length 1809 bp, 603 aa
Immediate neighbours sigA, antE
Sequences Protein DNA Advanced_DNA
Genetic context
DnaG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
DnaG expression.png















Categories containing this gene/protein

DNA replication, essential genes

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU25210

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • required for bacteriophage SPP1 replication PubMed
  • Protein family: DNA primase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • the enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´ PubMed
    • DnaG primase activity is stimulates by DnaC PubMed

Database entries

  • Structure: 1D0Q (zinc binding domain, Geobacillus stearothermophilus), 1Z8S (DnaB binding domain, AA 452-597, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´
  • Regulatory mechanism:
  • Additional information:
    • enzymatic activity is inhibited by (p)ppGpp during the ´stringent response´ PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews


Original publications