Difference between revisions of "Rnc"
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|style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites <br/>of the small cytoplasmic RNA precursor (''[[scr]]'') | |style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites <br/>of the small cytoplasmic RNA precursor (''[[scr]]'') | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15930 rnc] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]''' | |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]''' | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acpA]]'', ''[[smc]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acpA]]'', ''[[smc]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15930 Advanced_DNA] |
|- | |- | ||
|colspan="2" | '''Genetic context''' <br/> [[Image:rnc_context.gif]] | |colspan="2" | '''Genetic context''' <br/> [[Image:rnc_context.gif]] | ||
Revision as of 12:49, 13 May 2013
- Description: RNase III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria
| Gene name | rnc |
| Synonyms | rncS |
| Essential | yes |
| Product | endoribonuclease III |
| Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
| Gene expression levels in SubtiExpress: rnc | |
| Metabolic function and regulation of this protein in SubtiPathways: Protein secretion | |
| MW, pI | 28 kDa, 8.076 |
| Gene length, protein length | 747 bp, 249 aa |
| Immediate neighbours | acpA, smc |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
| |
Expression at a glance PubMed
| |
Contents
Categories containing this gene/protein
Rnases, translation, protein secretion, essential genes
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU15930
Phenotypes of a mutant
- essential PubMed
- the rnc gene can be deleted in strains cured of the Skin element and SPß prophage (or upon deletion of the toxin genes txpA and yonT) PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
- cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- degradation of txpA mRNA-RatA RNA duplex (encoded on the Skin element) PubMed
- degradation of yonT mRNA-as-yonT RNA duplex (encoded on the SP-beta prophage) PubMed
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- Structure:
- UniProt: P51833
- KEGG entry: [3]
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Ciaran Condon, IBPC, Paris, France Homepage
Your additional remarks
References
Reviews
Original publications
Durand S, Gilet L, Condon C The essential function of B. subtilis RNase III is to silence foreign toxin genes. PLoS Genet. 2012 8(12): e1003181. PubMed:23300471
