Difference between revisions of "SigM"

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|style="background:#ABCDEF;" align="center"|'''Function''' || responsible for intrinsic resistance against beta-lactam antibiotics  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || responsible for intrinsic resistance against beta-lactam antibiotics  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU09520 sigM]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU09520 sigM]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigM SigM]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigM SigM]
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdL]]'', ''[[yhdN]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhdL]]'', ''[[yhdN]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12791&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU09520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU09520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU09520 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:sigM_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:sigM_context.gif]]

Revision as of 12:26, 13 May 2013

Gene name sigM
Synonyms yhdM
Essential no
Product RNA polymerase ECF-type sigma factor SigM
Function responsible for intrinsic resistance against beta-lactam antibiotics
Gene expression levels in SubtiExpress: sigM
Interactions involving this protein in SubtInteract: SigM
MW, pI 19 kDa, 6.77
Gene length, protein length 489 bp, 163 aa
Immediate neighbours yhdL, yhdN
Sequences Protein DNA Advanced_DNA
Genetic context
SigM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigM expression.png















Categories containing this gene/protein

transcription, sigma factors and their control, cell envelope stress proteins (controlled by SigM, V, W, X, Y), resistance against toxins/ antibiotics

This gene is a member of the following regulons

SigM regulon

The SigM regulon

The gene

Basic information

  • Locus tag: BSU09520

Phenotypes of a mutant

  • SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
  • sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
  • increased sensitivity towards beta-lactam antibiotics PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family, ECF subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
    • shortage of available bactoprenol causes SigM activation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

John Helmann, Cornell University, USA Homepage

Your additional remarks

References

The SigM regulon

Other original publications